| Title : Crystallization and preliminary x-ray diffraction studies of a novel bacterial esterase - Bourne_1999_Acta.Crystallogr.D.Biol.Crystallogr_55_915 |
| Author(s) : Bourne PC , Isupov MN , Littlechild JA |
| Ref : Acta Crystallographica D Biol Crystallogr , 55 :915 , 1999 |
| Abstract : A novel bacterial esterase has been crystallized in two forms suitable for X-ray diffraction studies. Crystals have been obtained by vapour-phase diffusion at 290 K using ammonium sulfate as precipitant. The first crystals grew in space group C2 with unit-cell parameters a = 134.7, b = 55.8, c = 110.3 A, beta = 125.1 degrees. A monoclinic data set has been collected to 2.0 A resolution. Microseeding yielded a second crystal form which grew in space group P212121 with unit-cell parameters a = 57.1, b = 115.4, c = 130.4 A. Native data from these crystals have been collected to 1.6 A resolution. A molecular envelope has been determined using an uranyl acetate derivative for phase calculation. |
| ESTHER : Bourne_1999_Acta.Crystallogr.D.Biol.Crystallogr_55_915 |
| PubMedSearch : Bourne_1999_Acta.Crystallogr.D.Biol.Crystallogr_55_915 |
| PubMedID: 10089333 |
Bourne PC, Isupov MN, Littlechild JA (1999)
Crystallization and preliminary x-ray diffraction studies of a novel bacterial esterase
Acta Crystallographica D Biol Crystallogr
55 :915
Bourne PC, Isupov MN, Littlechild JA (1999)
Acta Crystallographica D Biol Crystallogr
55 :915