Braesel_2015_Chem.Biol_22_1325

Reference

Title : Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus - Braesel_2015_Chem.Biol_22_1325
Author(s) : Braesel J , Gotze S , Shah F , Heine D , Tauber J , Hertweck C , Tunlid A , Stallforth P , Hoffmeister D
Ref : Chemical Biology , 22 :1325 , 2015
Abstract :

The symbiotic fungus Paxillus involutus serves a critical role in maintaining forest ecosystems, which are carbon sinks of global importance. P. involutus produces involutin and other 2,5-diarylcyclopentenone pigments that presumably assist in the oxidative degradation of lignocellulose via Fenton chemistry. Their precise biosynthetic pathways, however, remain obscure. Using a combination of biochemical, genetic, and transcriptomic analyses, in addition to stable-isotope labeling with synthetic precursors, we show that atromentin is the key intermediate. Atromentin is made by tridomain synthetases of high similarity: InvA1, InvA2, and InvA5. An inactive atromentin synthetase, InvA3, gained activity after a domain swap that replaced its native thioesterase domain with that of InvA5. The found degree of multiplex biosynthetic capacity is unprecedented with fungi, and highlights the great importance of the metabolite for the producer.

PubMedSearch : Braesel_2015_Chem.Biol_22_1325
PubMedID: 26496685
Gene_locus related to this paper: paxin-inva2 , paxin-inva1 , paxin-inva3 , paxin-inva5 , paxin-inva6 , paxin-inva4

Related information

Gene_locus paxin-inva2    paxin-inva1    paxin-inva3    paxin-inva5    paxin-inva6    paxin-inva4

Citations formats

Braesel J, Gotze S, Shah F, Heine D, Tauber J, Hertweck C, Tunlid A, Stallforth P, Hoffmeister D (2015)
Three Redundant Synthetases Secure Redox-Active Pigment Production in the Basidiomycete Paxillus involutus
Chemical Biology 22 :1325

Braesel J, Gotze S, Shah F, Heine D, Tauber J, Hertweck C, Tunlid A, Stallforth P, Hoffmeister D (2015)
Chemical Biology 22 :1325