| Title : Adenosine triphosphatase activity associated with purified cholinergic synaptic vesicles of Torpedo marmorata - Breer_1977_Eur.J.Biochem_80_313 |
| Author(s) : Breer H , Morris SJ , Whittaker VP |
| Ref : European Journal of Biochemistry , 80 :313 , 1977 |
|
Abstract :
A rapid method for purifying Torpedo electric organ vesicles is described, which employs an isoosmotic continuous sucrose-glycine gradient followed by chromagography on CPG-10-3000 porous glass beads. The synaptic vesicles have a buoyant density of 1.057 g/ml. The purified vesicles are free of cholinesterase, lactate dehydrogenase and Na+, K+-stimulated ATPase activity. They contain a ouabaininsensitive, Na+, K+-inhibited, Mg2+, Ca2+-stimulated ATPase activity. This is further stimulated by acetylcholine but not by choline. |
| PubMedSearch : Breer_1977_Eur.J.Biochem_80_313 |
| PubMedID: 144598 |
Breer H, Morris SJ, Whittaker VP (1977)
Adenosine triphosphatase activity associated with purified cholinergic synaptic vesicles of Torpedo marmorata
European Journal of Biochemistry
80 :313
Breer H, Morris SJ, Whittaker VP (1977)
European Journal of Biochemistry
80 :313