| Title : [Estimation of the irreversible inhibition reaction rate constants and of the concentration of cholinesterase active sites under commensurable concentrations of enzyme and inhibitors] - Brestkin_1975_Biokhimiia_40_521 |
| Author(s) : Brestkin AP , Maizel EB , Smirnov OL |
| Ref : Biokhimiia , 40 :521 , 1975 |
|
Abstract :
Kinetic analysis of the interaction of butyrylcholinesterase and the phosphoorganic inhibitor GT-161 [(C2H2O)2P(O)SC2H4+N(CH3)2C6H5-1-] is carried out. Short time incubations of an enzyme and an inhibitor (1-3 sec), even under commensurable concentrations, were shown to enable the rate constants of irreversible enzyme inhibition to be calcualted by the formula for pseudomonomoleuclar reactions. A simple analytical method for the estimation of the enzyme active sites concentrations is proposed. |
| PubMedSearch : Brestkin_1975_Biokhimiia_40_521 |
| PubMedID: 1203370 |
Brestkin AP, Maizel EB, Smirnov OL (1975)
[Estimation of the irreversible inhibition reaction rate constants and of the concentration of cholinesterase active sites under commensurable concentrations of enzyme and inhibitors]
Biokhimiia
40 :521
Brestkin AP, Maizel EB, Smirnov OL (1975)
Biokhimiia
40 :521