Brestkin_1975_Zh.Evol.Biokhim.Fiziol_11_250

Reference

Title : [Acetylcholinesterase from the electric organ of the ray Torpedo marmorata] - Brestkin_1975_Zh.Evol.Biokhim.Fiziol_11_250
Author(s) : Brestkin AP , Grigor'eva GM , Eremeeva AM , Zhukovskii Iu G , Kuznetsova LR , Rakic L
Ref : Zhurnal Evoliutsionnoi Biokhimii i Fiziologii , 11 :250 , 1975
Abstract :

Studies have been made on substrate specificity of acetylcholinesterase (AChE;EC 3-1-1-7) from the electric organ of the ray T. marmorata with respect of choline and thiocholine esters, as well as on the effect of pH, salts and organophosphorus inhibitors (OPI) on the activity of the enzyme. Acetylcholine (ACh), propionycholine (PrCh) acetyl-beta-methylcholine (MeCh), acetylthiocholine ((ATCh) and propionylthiocholine (PrTCh) were hydrolyzed by the enzyme studied at the following relative rates-100: 28.8: 18.3: 87.2: 18.9 correspondingly. In all the cases, inhibition of the enzyme by high concentrations of the substrate was observed. As compared to other AChE, the enzyme from T. marmorata exhibits the highest affinity to ACh. For all the substrates studied, pH dependence of AChE activity followed the curve with maximum 7.5 for ACh and PrCh, 8.0-8.5 for ATCh and MeCh and 7.5-8.5 for PrTCh. Various salts (MgCl2), KCl, NaCl, NaBr, KI) increased AChE activity, the increase being the highest with MgCl2 (3.3 times) and NaCl (2.5X). Biomolecular rate constants ((k) II) for the interaction of AChE investigated with OPI containing cationic group-methylsulfomethylates, O-ethyl-S-(beta-ethylmercapto) ethylmethylthiophosphonate and O,O-diethyl-S-(beta-ethylmercapto) ethylthiophosphate, as well as methyl iodide O,O-disopropyl-S-(beta-phenylmethylamino) ethylphosphate-were significantly higher as compared with k(II) values for corresponding compounds without the cation. The value of k(II) sharply decreased with the increase in the size of the acyl radicals at phosphorus atom in the molecule of OPI.

PubMedSearch : Brestkin_1975_Zh.Evol.Biokhim.Fiziol_11_250
PubMedID: 3075

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Citations formats

Brestkin AP, Grigor'eva GM, Eremeeva AM, Zhukovskii Iu G, Kuznetsova LR, Rakic L (1975)
[Acetylcholinesterase from the electric organ of the ray Torpedo marmorata]
Zhurnal Evoliutsionnoi Biokhimii i Fiziologii 11 :250

Brestkin AP, Grigor'eva GM, Eremeeva AM, Zhukovskii Iu G, Kuznetsova LR, Rakic L (1975)
Zhurnal Evoliutsionnoi Biokhimii i Fiziologii 11 :250