Bronk_1995_Anal.Chem_67_2750

Reference

Title : Combined imaging and chemical sensing using a single optical imaging fiber - Bronk_1995_Anal.Chem_67_2750
Author(s) : Bronk KS , Michael KL , Pantano P , Walt DR
Ref : Analytical Chemistry , 67 :2750 , 1995
Abstract :

Despite many innovations and developments in the field of fiber-optic chemical sensors, optical fibers have not been employed to both view a sample and concurrently detect an analyte of interest. While chemical sensors employing a single optical fiber or a noncoherent fiberoptic bundle have been applied to a wide variety of analytical determinations, they cannot be used for imaging. Similarly, coherent imaging fibers have been employed only for their originally intended purpose, image transmission. We herein report a new technique for viewing a sample and measuring surface chemical concentrations that employs a coherent imaging fiber. The method is based on the deposition of a thin, analyte-sensitive polymer layer on the distal surface of a 350-microns-diameter imaging fiber. We present results from a pH sensor array and an acetylcholine biosensor array, each of which contains approximately 6000 optical sensors. The acetylcholine biosensor has a detection limit of 35 microM and a fast (< 1 s) response time. In association with an epifluorescence microscope and a charge-coupled device, these modified imaging fibers can display visual information of a remote sample with 4-microns spatial resolution, allowing for alternating acquisition of both chemical analysis and visual histology.

PubMedSearch : Bronk_1995_Anal.Chem_67_2750
PubMedID: 8779411

Related information

Citations formats

Bronk KS, Michael KL, Pantano P, Walt DR (1995)
Combined imaging and chemical sensing using a single optical imaging fiber
Analytical Chemistry 67 :2750

Bronk KS, Michael KL, Pantano P, Walt DR (1995)
Analytical Chemistry 67 :2750