| Title : Asymmetric synthesis of cis-3,5-diacetoxycyclopent-1-ene using metagenome-derived hydrolases - Brusehaber_2008_Tetrahedron.Asymmetry_19_730 |
| Author(s) : Brusehaber E , Bottcher D , Liebeton K , Eck J , Naumer C , Bornscheuer UT |
| Ref : Tetrahedron Asymmetry , 19 :730 , 2008 |
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Abstract :
Esterases and lipases originating from the metagenome (environmental DNA) were studied for the asymmetric hydrolysis of cis-3,5-diacetoxycyclopent-1-ene 1. Out of 83 enzymes, 35 were found to be active. Subsequent analytical and laboratory scale reactions identified three enzymes showing excellent (-)-preference and one enzyme with excellent (+)-selectivity exceeding even the performance of recombinant or commercial pig liver esterase, which is known as the best biocatalyst for this compound |
| PubMedSearch : Brusehaber_2008_Tetrahedron.Asymmetry_19_730 |
| PubMedID: |
Brusehaber E, Bottcher D, Liebeton K, Eck J, Naumer C, Bornscheuer UT (2008)
Asymmetric synthesis of cis-3,5-diacetoxycyclopent-1-ene using metagenome-derived hydrolases
Tetrahedron Asymmetry
19 :730
Brusehaber E, Bottcher D, Liebeton K, Eck J, Naumer C, Bornscheuer UT (2008)
Tetrahedron Asymmetry
19 :730