Brzin_1976_Histochem_48_283

Reference

Title : Iodide, thiocyanate and cyanide ions as capturing reagents in one-step copper-thiocholine method for cytochemical localization of cholinesterase activity - Brzin_1976_Histochem_48_283
Author(s) : Brzin M , Pucihar S
Ref : Histochemistry , 48 :283 , 1976
Abstract : The necessity of the presence of iodide in Cu-ThCh reaction was investigated by following the precipitate formation "in vitro" and by evaluating the ultrastructural localization of the precipitate in sympathetic ganglion cells of the frog and in the end-plate regions of the rat diaphragm. It was found that thiocyanate or cyanide is the only anion that can be substituted for iodide as the capturing agent in precipitation. The optimal concentration in the preincubation and incubation media of any one of the three anions is from 2 to 5 mM. At a concentration below 1 mM precipitation "in vitro" is considerably delayed as a result of which in electron microscopy diffusion artefacts appear in tissue sections. The unconverted primary precipitate obtained in the presence of iodide had been used for ultrastructural localization of ChE activity and now this use has been extended to precipitates obtained in the presence of CN- or CNS-. Better-quality localization in the presence of either one of the latter anions suggests that they, and particularly CN-, should be substituted for I- in the one-step Cu-ThCh method for the cytochemistry of cholinesterases.
ESTHER : Brzin_1976_Histochem_48_283
PubMedSearch : Brzin_1976_Histochem_48_283
PubMedID: 1022721

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Citations formats

Brzin M, Pucihar S (1976)
Iodide, thiocyanate and cyanide ions as capturing reagents in one-step copper-thiocholine method for cytochemical localization of cholinesterase activity
Histochemistry 48 :283

Brzin M, Pucihar S (1976)
Histochemistry 48 :283