Title : Measurement of acetylcholine and choline in brain by HPLC with electrochemical detection - Bymaster_1985_Life.Sci_37_1775 |
Author(s) : Bymaster FP , Perry KW , Wong DT |
Ref : Life Sciences , 37 :1775 , 1985 |
Abstract :
A simple and rapid method for measuring acetylcholine and choline using high performance liquid chromatography (HPLC) with electrochemical detection is presented. Acetylcholine and choline were first separated using reverse-phase chromatography; acetylcholine was then hydrolyzed post-column to choline by acetylcholinesterase. Choline was oxidized enzymatically by choline oxidase to betaine and hydrogen peroxide, and the peroxide was detected electrochemically. Changes in methodology from previous procedures include a different mobile phase, controlled heating of chromatography column and post-column reaction coil, and a different extraction method for quaternary amines. The changes resulted in less inhibition of derivatizing enzymes by mobile phase, narrow and consistent elution of peaks, and a rapid and efficient extraction of quaternary amines. Measurement of acetylcholine and choline in brain tissue was found to be replicable, and the levels agreed with literature values. |
PubMedSearch : Bymaster_1985_Life.Sci_37_1775 |
PubMedID: 3903413 |
Bymaster FP, Perry KW, Wong DT (1985)
Measurement of acetylcholine and choline in brain by HPLC with electrochemical detection
Life Sciences
37 :1775
Bymaster FP, Perry KW, Wong DT (1985)
Life Sciences
37 :1775