| Title : An immunochemical approach to the identification of the MBTA binding site of the nicotinic acetylcholine receptor of Torpedo californica - Cahill_1984_Biochem.Biophys.Res.Commun_122_602 |
| Author(s) : Cahill S , Schmidt J |
| Ref : Biochemical & Biophysical Research Communications , 122 :602 , 1984 |
|
Abstract :
Monospecific anti-[4-(N-maleimidobenzyl) trimethylammonium] (MBTA) antibodies were prepared from sera of rabbits immunized with an albumin-MBTA conjugate and used to synthesize an MBTA-specific immunosorbent. Torpedo californica acetylcholine receptor was affinity labeled with [3H]-MBTA and proteolyzed extensively with pronase, and the peptide fraction of the digest chromatographed on the anti-MBTA resin. The amino acid composition of the purified MBTA-peptide fraction was compared with the sequences flanking the seven cysteinyl residues of the alpha-subunit. The best fit was observed with the segment containing cysteine 142. |
| PubMedSearch : Cahill_1984_Biochem.Biophys.Res.Commun_122_602 |
| PubMedID: 6466328 |
Cahill S, Schmidt J (1984)
An immunochemical approach to the identification of the MBTA binding site of the nicotinic acetylcholine receptor of Torpedo californica
Biochemical & Biophysical Research Communications
122 :602
Cahill S, Schmidt J (1984)
Biochemical & Biophysical Research Communications
122 :602