Calakos_2023_Neuropsychopharmacology__

The cholinergic system is a critical mediator of cognition in animals. People who smoke cigarettes exhibit cognitive deficits, especially during quit attempts. Few studies jointly examine the cholinergic system and cognition in people while trying to quit smoking. We used positron emission tomography (PET) brain imaging with the beta(2)-subunit containing nicotinic acetylcholine receptor (beta(2)*-nAChR) partial agonist radioligand (-)-[(18)F]flubatine and the acetylcholinesterase inhibitor physostigmine to jointly examine the cholinergic system, smoking status, and cognition. (-)-[(18)F]Flubatine scans and cognitive data were acquired from twenty people who recently stopped smoking cigarettes (aged 38 +/- 11 years; 6 female, 14 male; abstinent 7 +/- 1 days) and 27 people who never smoked cigarettes (aged 29 +/- 8 years; 11 female, 16 male). A subset of fifteen recently abstinent smokers and 21 never smokers received a mid-scan physostigmine challenge to increase acetylcholine levels. Regional volume of distribution (V(T)) was estimated with equilibrium analysis at 'baseline' and post-physostigmine. Participants completed a cognitive battery prior to (-)-[(18)F]flubatine injection and physostigmine administration assessing executive function (Groton Maze Learning test), verbal learning (International Shopping List test), and working memory (One Back test). Physostigmine significantly decreased cortical (-)-[(18)F]flubatine V(T), consistent with increased cortical acetylcholine levels reducing the number of beta(2)*-nAChR sites available for (-)-[(18)F]flubatine binding, at comparable magnitudes across groups (p values < 0.05). A larger magnitude of physostigmine-induced decrease in (-)-[(18)F]flubatine V(T) was significantly associated with worse executive function in people who recently stopped smoking (p values < 0.05). These findings underscore the role of the cholinergic system in early smoking cessation and highlight the importance of neuroscience-informed treatment strategies.