| Title : The interaction between OPH and paraoxon at the air-water interface studied by AFM and epifluorescence microscopies - Cao_2005_Colloids.Surf.B.Biointerfaces_40_75 |
| Author(s) : Cao X , Mabrouki M , Mello SV , Leblanc RM , Rastogi VK , Cheng TC , DeFrank JJ |
| Ref : Colloids Surf B Biointerfaces , 40 :75 , 2005 |
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Abstract :
The paraoxon hydrolysis reaction catalyzed by organophosphorus hydrolase (OPH) monolayer at the air-water interface was studied. OPH-paraoxon interactions, occurring at the two-dimensional interface, by close-packed, highly orientated OPH monolayer, were investigated by several different surface chemistry techniques; e.g. surface pressure area isotherms, atomic force microscopy (AFM), and in situ epifluorescence microscopy. The characterization of OPH Langmuir and Langmuir-Blodgett films prepared in both the presence and absence of paraoxon, demonstrated significantly distinctive feature when compared with one another. Continuous growth of the OPH aggregates is a distinct phenomenon associated with hydrolysis, in addition to the pH changes in the local environment of the enzyme macromolecules. |
| PubMedSearch : Cao_2005_Colloids.Surf.B.Biointerfaces_40_75 |
| PubMedID: 15642457 |
Cao X, Mabrouki M, Mello SV, Leblanc RM, Rastogi VK, Cheng TC, DeFrank JJ (2005)
The interaction between OPH and paraoxon at the air-water interface studied by AFM and epifluorescence microscopies
Colloids Surf B Biointerfaces
40 :75
Cao X, Mabrouki M, Mello SV, Leblanc RM, Rastogi VK, Cheng TC, DeFrank JJ (2005)
Colloids Surf B Biointerfaces
40 :75