| Title : Enhancement of methanol resistance of Yarrowia lipolytica lipase 2 using beta-cyclodextrin as an additive: Insights from experiments and molecular dynamics simulation - Cao_2017_Enzyme.Microb.Technol_96_157 |
| Author(s) : Cao H , Jiang Y , Zhang H , Nie K , Lei M , Deng L , Wang F , Tan T |
| Ref : Enzyme Microb Technol , 96 :157 , 2017 |
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Abstract :
The methanol resistance of lipase is a critical parameter in enzymatic biodiesel production. In the present work, the methanol resistance of Yarrowia lipolytica Lipase 2 (YLLIP2) was significantly improved using beta-cyclodextrin (beta-CD) as an additive. According to the results, YLLIP2 with beta-CD exhibited approximately 7000U/mg specific activity in 30wt% methanol for 60min compared with no activity without beta-CD under the same conditions. Molecular dynamics (MD) simulation results indicated that the beta-CD molecules weakened the conformational change of YLLIP2 and maintained a semi-open state of the lid by overcoming the interference caused by methanol molecules. Furthermore, the beta-CD molecule could directly stabilize "pathway" regions (e.g., Asp61-Asp67) and indirectly stabilize "pathway" regions (e.g., Gly44-Phe50) by forming hydrogen bonds with "pathway" regions and nearby "pathway" regions, respectively. The regions stabilized by the beta-CD molecule then prevented the closure of active pockets, thus retaining the enzymatic activity of YLLIP2 with beta-CD in methanol solvent. |
| PubMedSearch : Cao_2017_Enzyme.Microb.Technol_96_157 |
| PubMedID: 27871377 |
Cao H, Jiang Y, Zhang H, Nie K, Lei M, Deng L, Wang F, Tan T (2017)
Enhancement of methanol resistance of Yarrowia lipolytica lipase 2 using beta-cyclodextrin as an additive: Insights from experiments and molecular dynamics simulation
Enzyme Microb Technol
96 :157
Cao H, Jiang Y, Zhang H, Nie K, Lei M, Deng L, Wang F, Tan T (2017)
Enzyme Microb Technol
96 :157