| Title : Postsynaptic effects of the phorbol ester TPA on frog end-plates - Caratsch_1986_Pflugers.Arch_407_409 |
| Author(s) : Caratsch CG , Grassi F , Molinaro M , Eusebi F |
| Ref : Pflugers Arch , 407 :409 , 1986 |
| Abstract : The effects of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), a specific activator of protein kinase C (PKc), were examined on the frog neuromuscular junction. The depolarization elicited by iontophoretically applied acetylcholine (ACh) was reversibly decreased by 20-60% when muscle fibres were exposed to 1-5 X 10(-7) M TPA. Liposome-delivered phosphatidylcholine (100 micrograms/ml) prevented this effect. A similar decrease in ACh-sensitivity was produced by diacylglycerol (diolein), a physiological activator of PKc, but in this case the decrease was only partially reversible. In TPA-Ringer, the peak size of miniature end-plate potentials exhibited a small decrease; miniature end-plate currents were reduced in size and their decay time constant became longer and relatively independent of membrane potential. The possibility that these TPA-induced actions are mediated by activation of PKc is discussed. |
| ESTHER : Caratsch_1986_Pflugers.Arch_407_409 |
| PubMedSearch : Caratsch_1986_Pflugers.Arch_407_409 |
| PubMedID: 3774508 |
Caratsch CG, Grassi F, Molinaro M, Eusebi F (1986)
Postsynaptic effects of the phorbol ester TPA on frog end-plates
Pflugers Arch
407 :409
Caratsch CG, Grassi F, Molinaro M, Eusebi F (1986)
Pflugers Arch
407 :409