Cardullo_2024_J.Sci.Food.Agric__

Reference

Title : Flavonoids with lipase inhibitory activity from lemon squeezing waste: isolation, multispectroscopic and in silico studies - Cardullo_2024_J.Sci.Food.Agric__
Author(s) : Cardullo N , Calcagno D , Pulvirenti L , Sciacca C , Pittala MGG , Maccarronello AE , Thevenard F , Muccilli V
Ref : J Sci Food Agric , : , 2024
Abstract :

BACKGROUND: Obesity is recognized as a lifestyle-related disease and the main risk factor for a series of pathological conditions, including cardiovascular diseases, hypertension and type 2 diabetes. Citrus limon is an important medicinal plant, and its fruits are rich in flavonoids investigated for their potential in managing obesity. In the present work, a green extraction applied to lemon squeezing waste (LSW) was optimized to recover pancreatic lipase (PL) inhibitors. RESULTS: The microwave-assisted procedure yielded an extract with higher lipase inhibitory activity than those obtained by maceration and ultrasound. The main compounds present in the extract were identified by high-performance liquid chromatographic-mass spectrometric analysis, and hesperidin, eriocitrin and 4'-methyllucenin II were isolated. The three compounds were evaluated for in vitro PL inhibitory activity, and 4'-methyllucenin II resulted in the most promising inhibitor (IC(50) = 12.1 micromol L(-1); K(i) = 62.2 micromol L(-1)). Multispectroscopic approaches suggested the three flavonoids act as competitive inhibitors and the binding studies indicated a greater interaction between PL and 4'-methyllucenin II. Docking analysis indicated the significant interactions of the three flavonoids with the PL catalytic site. CONCLUSION: The present work highlights flavonoid glycosides as promising PL inhibitors and proposes LSW as a safe ingredient for the preparation of food supplements for managing obesity. 2024 Society of Chemical Industry.

PubMedSearch : Cardullo_2024_J.Sci.Food.Agric__
PubMedID: 38775623

Related information

Citations formats

Cardullo N, Calcagno D, Pulvirenti L, Sciacca C, Pittala MGG, Maccarronello AE, Thevenard F, Muccilli V (2024)
Flavonoids with lipase inhibitory activity from lemon squeezing waste: isolation, multispectroscopic and in silico studies
J Sci Food Agric :

Cardullo N, Calcagno D, Pulvirenti L, Sciacca C, Pittala MGG, Maccarronello AE, Thevenard F, Muccilli V (2024)
J Sci Food Agric :