Title : Several targets involved in Alzheimer's disease amyloidogenesis are affected by morin and isoquercitrin - Carmona_2018_Nutr.Neurosci__1 |
Author(s) : Carmona V , Martin-Aragon S , Goldberg J , Schubert D , Bermejo-Bescos P |
Ref : Nutr Neurosci , :1 , 2018 |
Abstract :
Long-term consumption of phytochemicals has been associated with a decreased risk of dementia. The modes of action of two flavonols (morin and isoquercitrin) and two flavanones (hesperidin and neohesperidin) were characterized as single-compound drugs in several Alzheimer's disease (AD)-related assays. First, these phytochemicals were assayed in an amyloid toxicity model (MC65 cells). Second, we examined the activity of the flavonoids in cell-free assays against beta- and gamma-secretases and acetylcholinesterase activities, as well as agents able to modify the fibrillogenesis of the amyloid beta-peptide. Additionally, they were assayed against glutamate-induced oxytosis, as scavengers of reactive oxygen species (ROS), as inhibitors of caspase-3, -8 and -9 activation and as modulators of the chymotrypsin-like activity of the ubiquitin-proteasome system. Morin and isoquercitrin, unlike flavanones, exhibited significant activities as beta- and gamma-secretase inhibitors, as well as capacity to inhibit Abeta aggregation and favor its disaggregation. Flavonols and flavanones showed ROS scavenger activity (P < 0.05), attenuation of caspase-3 and -9 activation (P < 0.05) and restoration of the reduced chymotrypsin-like activity of proteasome 20S (P < 0.05) upon H2O2 exposure of APPswe cells. Flavanones failed to protect against glutamate-induced oxytosis. These findings provide new insight into the anti-amyloidogenic effects of morin and isoquercitrin. |
PubMedSearch : Carmona_2018_Nutr.Neurosci__1 |
PubMedID: 30326823 |
Carmona V, Martin-Aragon S, Goldberg J, Schubert D, Bermejo-Bescos P (2018)
Several targets involved in Alzheimer's disease amyloidogenesis are affected by morin and isoquercitrin
Nutr Neurosci
:1
Carmona V, Martin-Aragon S, Goldberg J, Schubert D, Bermejo-Bescos P (2018)
Nutr Neurosci
:1