Carvalho_2013_PLoS.One_8_e62268

Reference

Title : Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda - Carvalho_2013_PLoS.One_8_e62268
Author(s) : Carvalho RA , Omoto C , Field LM , Williamson MS , Bass C
Ref : PLoS ONE , 8 :e62268 , 2013
Abstract :

The fall armyworm Spodoptera frugiperda is an economically important pest of small grain crops that occurs in all maize growing regions of the Americas. The intensive use of chemical pesticides for its control has led to the selection of resistant populations, however, to date, the molecular mechanisms underlying resistance have not been characterised. In this study the mechanisms involved in the resistance of two S. frugiperda strains collected in Brazil to chlorpyrifos (OP strain) or lambda-cyhalothrin (PYR strain) were investigated using molecular and genomic approaches. To examine the possible role of target-site insensitivity the genes encoding the organophosphate (acetylcholinesterase, AChE) and pyrethroid (voltage-gated sodium channel, VGSC) target-site proteins were PCR amplified. Sequencing of the S. frugiperda ace-1 gene identified several nucleotide changes in the OP strain when compared to a susceptible reference strain (SUS). These result in three amino acid substitutions, A201S, G227A and F290V, that have all been shown previously to confer organophosphate resistance in several other insect species. Sequencing of the gene encoding the VGSC in the PYR strain, identified mutations that result in three amino acid substitutions, T929I, L932F and L1014F, all of which have been shown previously to confer knockdown/super knockdown-type resistance in several arthropod species. To investigate the possible role of metabolic detoxification in the resistant phenotype of the OP and PYR stains all EST sequences available for S. frugiperda were used to design a gene-expression microarray. This was then used to compare gene expression in the resistant strains with the susceptible reference strain. Members of several gene families, previously implicated in metabolic resistance in other insects were found to be overexpressed in the resistant strains including glutathione S-transferases, cytochrome P450s and carboxylesterases. Taken together these results provide evidence that both target-site and metabolic mechanisms underlie the resistance of S. frugiperda to pyrethroids and organophosphates.

PubMedSearch : Carvalho_2013_PLoS.One_8_e62268
PubMedID: 23614047
Gene_locus related to this paper: spolt-ACHE1

Related information

Mutation G227A_spolt-ACHE1    A201S_spolt-ACHE1    F290V_spolt-ACHE1
Gene_locus spolt-ACHE1
Gene_locus_frgt spofr-n0a2k3

Citations formats

Carvalho RA, Omoto C, Field LM, Williamson MS, Bass C (2013)
Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda
PLoS ONE 8 :e62268

Carvalho RA, Omoto C, Field LM, Williamson MS, Bass C (2013)
PLoS ONE 8 :e62268