| Title : Stimulation of acetylcholine output from brain slices caused by the ionophores BrX-537A and A23187 - Casamenti_1978_Br.J.Pharmacol_63_259 |
| Author(s) : Casamenti F , Mantovani P , Pepeu G |
| Ref : British Journal of Pharmacology , 63 :259 , 1978 |
| Abstract : 1 The effect of two ionophores, BrX-537A (Bromolasolacid) and A 23187, on acetylcholine (ACh) output from brain slices was studied. 2 The slices were prepared from rat cerebral cortex, incubated in Krebs solution containing physostigmine and ACh output determined by bioassay. 3 Both ionophores enhanced ACh output. BrX-537A exerted its maximal effect, a six fold increase, at a concentration of 1.8 micron, while A 23187 caused a three fold increase at a concentration of 58 micron. 4 When the slices were incubated in a Ca-free medium, the effect of A 23187 on ACh output was only reduced, BrX-537A was abolished while that of BrX-537A was also active when disodium edetate (EDTA) was added to the the Ca-free medium. 5 The activity of BrX-537A was not affected by the presence of tetrodotoxin in the incubation medium. 6 The stimulation of ACh output elicited by KCl (25 mM) was increased further by hyoscine, but not by BrX-537A. Hyoscine however had no effect when ACh output was stimulated by BrX-537A. 7 The effect of BrX-537A on ACh output was potentiated by the addition of Mg2+ (9.3 mM) to the incubation medium and was reduced in a Mg-free medium. 8 It is concluded that A 23187 stimulates ACh output by transporting extracellular Ca2+ into cholinergic nerve endings. The effect of BrX-537A does not depend only on Ca2+ but also on other mechanisms. |
| ESTHER : Casamenti_1978_Br.J.Pharmacol_63_259 |
| PubMedSearch : Casamenti_1978_Br.J.Pharmacol_63_259 |
| PubMedID: 96898 |
Casamenti F, Mantovani P, Pepeu G (1978)
Stimulation of acetylcholine output from brain slices caused by the ionophores BrX-537A and A23187
British Journal of Pharmacology
63 :259
Casamenti F, Mantovani P, Pepeu G (1978)
British Journal of Pharmacology
63 :259