Cassanelli_2006_Insect.Biochem.Mol.Biol_36_642

Reference

Title : Acetylcholinesterase mutation in an insecticide-resistant population of the codling moth Cydia pomonella (L.) - Cassanelli_2006_Insect.Biochem.Mol.Biol_36_642
Author(s) : Cassanelli S , Reyes M , Rault M , Carlo Manicardi G , Sauphanor B
Ref : Insect Biochemistry & Molecular Biology , 36 :642 , 2006
Abstract :

Two strains of Cydia pomonella (L.) (Lepidoptera: Tortricidae) were selected in the lab by exposure to increasing concentrations of diflubenzuron (Rdfb strain) or azinphos-methyl (Raz strain). Insecticide bioassays showed that the adults of the Rdfb strain exhibited a 2.6-fold and a 7.7-fold resistance ratio to azinphos-methyl and carbaryl, respectively compared to a susceptible strain (S) whereas the adults of the Raz strain exhibited a 6.7-fold resistance ratio to azinphos-methyl and a 130-fold resistance ratio to carbaryl. In the Raz strain, a target site resistance mechanism was suggested by the inhibition of acetylcholinesterase (AChE) activity. In fact the ki values did not discriminate the S and Rdfb strains, while the Raz strain exhibited a 1.7-fold and a 14-fold increase in ki value compared to the S strain for azinphos-methyl oxon and carbaryl, respectively. To verify this hypothesis, two cloned AChE cDNAs sequences (named cydpom-ace2 e cydpom-ace1) were compared between the susceptible and the resistant strains. No difference in the deduced amino acid sequence was found in cydpom-ace2 (orthologous to the Drosophila melanogaster AChE). In the putative cydpom-ace1 (paralogous to the Drosophila AChE), a single amino acid substitution F399V was exclusively present in the Raz strain. The F399 lined the active site of the enzyme and the F399V substitution likely could influence the accessibility of different types of inhibitors to the catalytic site of the insensitive cydpom-ace1.

PubMedSearch : Cassanelli_2006_Insect.Biochem.Mol.Biol_36_642
PubMedID: 16876707
Gene_locus related to this paper: cydpo-ACHE1 , cydpo-ACHE2

Related information

Mutation F399V_cydpo-ACHE1
Gene_locus F399V_cydpo-ACHE1    cydpo-ACHE1    cydpo-ACHE2

Citations formats

Cassanelli S, Reyes M, Rault M, Carlo Manicardi G, Sauphanor B (2006)
Acetylcholinesterase mutation in an insecticide-resistant population of the codling moth Cydia pomonella (L.)
Insect Biochemistry & Molecular Biology 36 :642

Cassanelli S, Reyes M, Rault M, Carlo Manicardi G, Sauphanor B (2006)
Insect Biochemistry & Molecular Biology 36 :642