| Title : Cytotoxicity and genotoxicity of DMABEE, a co-photoinitiator of resin polymerization, on CHO-K1 cells: Role of redox and carboxylesterase - Chang_2020_J.Biomed.Mater.Res.B.Appl.Biomater_108_2088 |
| Author(s) : Chang HH , Shih WC , Wang YL , Tsai YL , Chen YJ , Chang MC , Jeng JH |
| Ref : J Biomed Mater Res B Appl Biomater , 108 :2088 , 2020 |
| Abstract : The 4-dimethylaminobenzoic acid ethyl ester (DMABEE) is an important co-initiator for resin polymerization in dental resinous materials. As a radical forming chemical with high lipophilicity, the genotoxicity and cytotoxicity of DMABEE deserve prudent investigation. In this study, we found that DMABEE reduced the viability and proliferation of Chinese hamster ovary (CHO-K1) cells in a dose-dependent manner, and altered cell morphology at higher concentrations. G0/G1 cell cycle arrest was induced by DMABEE at 0.25-0.75 mM, and cell proportion of sub-G0/G1 phase was significantly elevated at 1 mM while cell apoptosis was observed. Genotoxic effect was noted when cells were treated by 0.1 mM DMABEE, as revealed by increase of micronucleus formation. Reactive oxygen species overproduction was observed as cells treated with 0.75 and 1 mM, while elevation of intracellular glutathione was noticeable since 0.1 mM. Contrary to our expectation, pretreatment by N-acetyl-l-cysteine enhanced the toxicity of DMABEE on CHO-K1 cells. Catalase mildly reduced the toxic effect and carboxylesterase showed obvious ability to reverse the toxicity of DMABEE. These findings highlight the mechanism of DMABEE toxicity and provide clues for safety improvement of its application in clinical dental treatment. |
| ESTHER : Chang_2020_J.Biomed.Mater.Res.B.Appl.Biomater_108_2088 |
| PubMedSearch : Chang_2020_J.Biomed.Mater.Res.B.Appl.Biomater_108_2088 |
| PubMedID: 31880385 |
Chang HH, Shih WC, Wang YL, Tsai YL, Chen YJ, Chang MC, Jeng JH (2020)
Cytotoxicity and genotoxicity of DMABEE, a co-photoinitiator of resin polymerization, on CHO-K1 cells: Role of redox and carboxylesterase
J Biomed Mater Res B Appl Biomater
108 :2088
Chang HH, Shih WC, Wang YL, Tsai YL, Chen YJ, Chang MC, Jeng JH (2020)
J Biomed Mater Res B Appl Biomater
108 :2088