| Title : Identification of thermophilic bacterial strains producing thermotolerant hydrolytic enzymes from manure compost - Charbonneau_2012_Indian.J.Microbiol_52_41 |
| Author(s) : Charbonneau DM , Meddeb-Mouelhi F , Boissinot M , Sirois M , Beauregard M |
| Ref : Indian J Microbiol , 52 :41 , 2012 |
|
Abstract :
Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: Geobacillus thermodenitrificans, Bacillus smithii, Ureibacillus suwonensis and Aneurinibacillus thermoaerophilus. PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the G. thermodenitrificans isolates. Isolates were screened for thermotolerant hydrolytic activities (60-65 degrees C). Thermotolerant lipolytic activities were detected for G. thermodenitrificans, A. thermoaerophilus and B. smithii. Thermotolerant protease, alpha-amylase and xylanase activities were also observed in the G. thermodenitrificans group. These species represent a source of potential novel thermostable enzymes for industrial applications. |
| PubMedSearch : Charbonneau_2012_Indian.J.Microbiol_52_41 |
| PubMedID: 23448754 |
Charbonneau DM, Meddeb-Mouelhi F, Boissinot M, Sirois M, Beauregard M (2012)
Identification of thermophilic bacterial strains producing thermotolerant hydrolytic enzymes from manure compost
Indian J Microbiol
52 :41
Charbonneau DM, Meddeb-Mouelhi F, Boissinot M, Sirois M, Beauregard M (2012)
Indian J Microbiol
52 :41