| Title : A method to estimate acetylcholinesterase-active sites and turnover in insects - Charpentier_2000_Anal.Biochem_285_76 |
| Author(s) : Charpentier A , Menozzi P , Marcel V , Villatte F , Fournier D |
| Ref : Analytical Biochemistry , 285 :76 , 2000 |
|
Abstract :
Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy)-1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific. |
| PubMedSearch : Charpentier_2000_Anal.Biochem_285_76 |
| PubMedID: 10998265 |
Charpentier A, Menozzi P, Marcel V, Villatte F, Fournier D (2000)
A method to estimate acetylcholinesterase-active sites and turnover in insects
Analytical Biochemistry
285 :76
Charpentier A, Menozzi P, Marcel V, Villatte F, Fournier D (2000)
Analytical Biochemistry
285 :76