| Title : Polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas stutzeri 1317 had different substrate specificities - Chen_2004_FEMS.Microbiol.Lett_234_231 |
| Author(s) : Chen JY , Liu T , Zheng Z , Chen JC , Chen GQ |
| Ref : FEMS Microbiology Letters , 234 :231 , 2004 |
|
Abstract :
The whole polyhydroxyalkanoate (PHA) synthesis gene locus of Pseudomonas stutzeri strain 1317 containing PHA synthase genes phaC1Ps, phaC2Ps and PHA depolymerase gene phaZPs was cloned using a PCR cloning strategy. The sequence analysis results of the phaC1Ps, phaC2Ps and phaZPs showed high homology to the corresponding pha loci of the known Pseudomonas strains, respectively. PhaC1Ps and PhaC2Ps were functionally expressed in recombinant Escherichia coli strains and their substrate specificity was compared. The results demonstrated that PhaC1Ps and PhaC2Ps from P. stutzeri 1317 had different substrate specificities when expressed in E. coli. In details, PhaC2Ps could incorporate both short-chain-length 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates (mcl 3HA) into PHA, while PhaC1Ps only favored mcl 3HA for polymerization. |
| PubMedSearch : Chen_2004_FEMS.Microbiol.Lett_234_231 |
| PubMedID: 15135527 |
| Gene_locus related to this paper: pseme-PHAC1 , pseme-PHAZ , psest-PHAC2 |
| Gene_locus | pseme-PHAC1 pseme-PHAZ psest-PHAC2 |
Chen JY, Liu T, Zheng Z, Chen JC, Chen GQ (2004)
Polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas stutzeri 1317 had different substrate specificities
FEMS Microbiology Letters
234 :231
Chen JY, Liu T, Zheng Z, Chen JC, Chen GQ (2004)
FEMS Microbiology Letters
234 :231