Chen_2009_J.Biol.Chem_284_10627

Reference

Title : Characterization of the polyoxin biosynthetic gene cluster from Streptomyces cacaoi and engineered production of polyoxin H - Chen_2009_J.Biol.Chem_284_10627
Author(s) : Chen W , Huang T , He X , Meng Q , You D , Bai L , Li J , Wu M , Li R , Xie Z , Zhou H , Zhou X , Tan H , Deng Z
Ref : Journal of Biological Chemistry , 284 :10627 , 2009
Abstract :

A gene cluster (pol) essential for the biosynthesis of polyoxin, a nucleoside antibiotic widely used for the control of phytopathogenic fungi, was cloned from Streptomyces cacaoi. A 46,066-bp region was sequenced, and 20 of 39 of the putative open reading frames were defined as necessary for polyoxin biosynthesis as evidenced by its production in a heterologous host, Streptomyces lividans TK24. The role of PolO and PolA in polyoxin synthesis was demonstrated by in vivo experiments, and their functions were unambiguously characterized as O-carbamoyltransferase and UMP-enolpyruvyltransferase, respectively, by in vitro experiments, which enabled the production of a modified compound differing slightly from that proposed earlier. These studies should provide a solid foundation for the elucidation of the molecular mechanisms for polyoxin biosynthesis, and set the stage for combinatorial biosynthesis using genes encoding different pathways for nucleoside antibiotics.

PubMedSearch : Chen_2009_J.Biol.Chem_284_10627
PubMedID: 19233844
Gene_locus related to this paper: strci-c1ic18

Related information

Gene_locus strci-c1ic18

Citations formats

Chen W, Huang T, He X, Meng Q, You D, Bai L, Li J, Wu M, Li R, Xie Z, Zhou H, Zhou X, Tan H, Deng Z (2009)
Characterization of the polyoxin biosynthetic gene cluster from Streptomyces cacaoi and engineered production of polyoxin H
Journal of Biological Chemistry 284 :10627

Chen W, Huang T, He X, Meng Q, You D, Bai L, Li J, Wu M, Li R, Xie Z, Zhou H, Zhou X, Tan H, Deng Z (2009)
Journal of Biological Chemistry 284 :10627