Title : Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG\/ENaC mechanosensory proteins - Chen_2016_Mol.Biol.Cell_27_1272 |
Author(s) : Chen Y , Bharill S , Altun Z , O'Hagan R , Coblitz B , Isacoff EY , Chalfie M |
Ref : Mol Biology of the cell , 27 :1272 , 2016 |
Abstract :
Caenorhabditis eleganssenses gentle touch via a mechanotransduction channel formed from the DEG/ENaC proteins MEC-4 and MEC-10. An additional protein, the paraoxonase-like protein MEC-6, is essential for transduction, and previous work suggested that MEC-6 was part of the transduction complex. We found that MEC-6 and a similar protein, POML-1, reside primarily in the endoplasmic reticulum and do not colocalize with MEC-4 on the plasma membrane in vivo. As with MEC-6, POML-1 is needed for touch sensitivity, the neurodegeneration caused by themec-4(d)mutation, and the expression and distribution of MEC-4 in vivo. Both proteins are likely needed for the proper folding or assembly of MEC-4 channels in vivo as measured by FRET. MEC-6 detectably increases the rate of MEC-4 accumulation on theXenopusoocyte plasma membrane. These results suggest that MEC-6 and POML-1 interact with MEC-4 to facilitate expression and localization of MEC-4 on the cell surface. Thus MEC-6 and POML-1 act more like chaperones for MEC-4 than channel components. |
PubMedSearch : Chen_2016_Mol.Biol.Cell_27_1272 |
PubMedID: 26941331 |
Chen Y, Bharill S, Altun Z, O'Hagan R, Coblitz B, Isacoff EY, Chalfie M (2016)
Caenorhabditis elegans paraoxonase-like proteins control the functional expression of DEG\/ENaC mechanosensory proteins
Mol Biology of the cell
27 :1272
Chen Y, Bharill S, Altun Z, O'Hagan R, Coblitz B, Isacoff EY, Chalfie M (2016)
Mol Biology of the cell
27 :1272