Chen_2017_Molecules_22_E795

It is difficult to screen out as many active components as possible from natural plants all at one time. In this study, subfractions of Forsythia suspensa leaves were firstly prepared; then, their inhibitive abilities on pancreatic lipase were tested; finally, the highest inhibiting subfraction was screened by self-made immobilized pancreatic lipase. Results showed that nine ligands, including eight inhibitors and one promotor, were screened out all at one time. They were three flavonoids (rutin, IC50: 149 +/- 6.0 mumol/L; hesperidin, 52.4 mumol/L; kaempferol-3-O-rutinoside, isolated from F. suspensa leaves for the first time, IC50 notably reached 2.9 +/- 0.5 mumol/L), two polyphenols (chlorogenic acid, 3150 +/- 120 mumol/L; caffeic acid, 1394 +/- 52 mumol/L), two lignans (phillyrin, promoter; arctigenin, 2129 +/- 10.5 mumol/L), and two phenethyl alcohol (forsythiaside A, 2155 +/- 8.5 mumol/L; its isomer). Their action mechanisms included competitive inhibition, competitive promotion, noncompetitive inhibition, and uncompetitive inhibition. In sum, using the appropriate methods, more active ingredients can be simply and quickly screened out all at one time from a complex natural product system. In addition, F. suspensa leaves contain numerous inhibitors of pancreatic lipase.