| Title : Effects of Huangqi (Hex) on Inducing Cell Differentiation and Cell Death in K562 and HEL Cells - Cheng_2004_Acta.Biochim.Biophys.Sin.(Shanghai)_36_211 |
| Author(s) : Cheng XD , Hou CH , Zhang XJ , Xie HY , Zhou WY , Yang L , Zhang SB , Qian RL |
| Ref : Acta Biochim Biophys Sin (Shanghai) , 36 :211 , 2004 |
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Abstract :
Huangqi (Astragalus membranaceus), a traditional Chinese medicine, has been used to ameliorate side effects of cancer chemotherapy in China. However, little is known about its molecular mechanisms. Here we show that induction of K562 or HEL cells with 1.5 mg/ml of Huangqi (Hex) (Components extracted from Huangqi) for 3-5 d results in the expression of beta-globin gene in both cell lines and leads to terminal differentiation. Moreover, the apoptosis in HEL cells can be induced by increasing concentration of Huangqi (Hex) to 4.5 mg/ml for 3-5 d. Upregulation of Apaf-1, caspase-3 and acetylcholinesterase (AChE) in HEL cells may play a crucial role in the process of apoptosis. The prospect of inducing expression of adult (beta) globin gene and apoptosis selectively in cancer cells is obviously attractive from a therapeutic point of view. |
| PubMedSearch : Cheng_2004_Acta.Biochim.Biophys.Sin.(Shanghai)_36_211 |
| PubMedID: 15202506 |
Cheng XD, Hou CH, Zhang XJ, Xie HY, Zhou WY, Yang L, Zhang SB, Qian RL (2004)
Effects of Huangqi (Hex) on Inducing Cell Differentiation and Cell Death in K562 and HEL Cells
Acta Biochim Biophys Sin (Shanghai)
36 :211
Cheng XD, Hou CH, Zhang XJ, Xie HY, Zhou WY, Yang L, Zhang SB, Qian RL (2004)
Acta Biochim Biophys Sin (Shanghai)
36 :211