Title : Carbamoylcholine and gastrin induce inositol lipid turnover in canine gastric parietal cells - Chiba_1988_Am.J.Physiol_255_G99 |
Author(s) : Chiba T , Fisher SK , Park J , Seguin EB , Agranoff BW , Yamada T |
Ref : American Journal of Physiology , 255 :G99 , 1988 |
Abstract :
The potential role of inositol phospholipid turnover in mediating acid secretion was examined in a preparation enriched for isolated canine gastric parietal cells. The stimulatory effects of carbamoylcholine (carbachol) and gastrin on parietal cell uptake of [14C]aminopyrine were linked to dose- and time-dependent selective reduction in cellular phosphatidylinositol content, although the specific fatty acid composition of the phosphoinositides was not altered. Analysis of [3H]inositol phosphates accumulated in cells prelabeled with [3H]inositol revealed an increase in labeled inositol trisphosphate by 5 min of incubation with either carbachol or gastrin. Furthermore, after preincubation of parietal cells in medium containing [32P]orthophosphate, the two secretagogues elicited a time-dependent decrease in 32P labeling of phosphatidylinositol 4,5-bisphosphate and concomitant increase in labeling of phosphatidic acid. These data demonstrate that the acid secretagogue actions of carbachol and gastrin are correlated with turnover of cellular inositol phospholipids in a preparation consisting predominantly of parietal cells. |
PubMedSearch : Chiba_1988_Am.J.Physiol_255_G99 |
PubMedID: 2839045 |
Chiba T, Fisher SK, Park J, Seguin EB, Agranoff BW, Yamada T (1988)
Carbamoylcholine and gastrin induce inositol lipid turnover in canine gastric parietal cells
American Journal of Physiology
255 :G99
Chiba T, Fisher SK, Park J, Seguin EB, Agranoff BW, Yamada T (1988)
American Journal of Physiology
255 :G99