Title : Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 - Choi_2021_Polymers.(Basel)_13_ |
Author(s) : Choi TR , Park YL , Song HS , Lee SM , Park SL , Lee HS , Kim HJ , Bhatia SK , Gurav R , Choi KY , Lee YK , Yang YH |
Ref : Polymers (Basel) , 13 : , 2021 |
Abstract :
Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes (phaC). Pseudomonas sp. B14-6 produced poly(6% 3-hydroxybutyrate-co-94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl-co-mcl PHA determined a glass transition temperature (T(g)) of 15.3 degreesC, melting temperature of 112.8 degreesC, crystallization temperature of 86.8 degreesC, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 x 10(4), weight average molecular weight of 9.1 x 10(4), and polydispersity index value of 2.5. Overall, the novel Pseudomonas sp. B14-6 produced a polymer with high medium-chain-length content, low T(g), and low crystallinity, indicating its potential use in medical applications. |
PubMedSearch : Choi_2021_Polymers.(Basel)_13_ |
PubMedID: 33925903 |
Choi TR, Park YL, Song HS, Lee SM, Park SL, Lee HS, Kim HJ, Bhatia SK, Gurav R, Choi KY, Lee YK, Yang YH (2021)
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6
Polymers (Basel)
13 :
Choi TR, Park YL, Song HS, Lee SM, Park SL, Lee HS, Kim HJ, Bhatia SK, Gurav R, Choi KY, Lee YK, Yang YH (2021)
Polymers (Basel)
13 :