Clery_1995_Biochim.Biophys.Acta_1253_85

Reference

Title : Kinetics of butyrylcholinesterase in reversed micelles under high pressure - Clery_1995_Biochim.Biophys.Acta_1253_85
Author(s) : Clery C , Bec N , Balny C , Mozhaev VV , Masson P
Ref : Biochimica & Biophysica Acta , 1253 :85 , 1995
Abstract :

The combined effects of high pressure and reversed micelles have been studied to modulate the catalytic behaviour of butyrylcholinesterase. The purpose of this study was to determine whether the conformational plasticity of the enzyme is altered by entrapment in reversed micelles. The presence of soman, an irreversible inhibitor of cholinesterase was used to bring to the fore a possible modification of the enzyme behaviour in this system under pressure. Results show differences between enzyme in conventional medium and in reversed micelles regarding the mechanism of BCHE catalyzed hydrolysis of acetylthiocholine. In both systems, the enzyme displays a non-Michaelian behaviour with this substrate. In conventional medium the kinetics is multiphasic with an activation phase followed by an inhibition phase at high concentration. In reversed micelles there is inhibition by excess substrate but the activation phase is missing. This behaviour may be the result of a change of the enzyme conformational plasticity when is entrapped in reversed micelles.

PubMedSearch : Clery_1995_Biochim.Biophys.Acta_1253_85
PubMedID: 7492604

Related information

Citations formats

Clery C, Bec N, Balny C, Mozhaev VV, Masson P (1995)
Kinetics of butyrylcholinesterase in reversed micelles under high pressure
Biochimica & Biophysica Acta 1253 :85

Clery C, Bec N, Balny C, Mozhaev VV, Masson P (1995)
Biochimica & Biophysica Acta 1253 :85