| Title : Comparison of 10,11-Dehydrocurvularin Polyketide Synthases from Alternaria cinerariae and Aspergillus terreus Highlights Key Structural Motifs - Cochrane_2015_Chembiochem_16_2479 |
| Author(s) : Cochrane RV , Gao Z , Lambkin GR , Xu W , Winter JM , Marcus SL , Tang Y , Vederas JC |
| Ref : Chembiochem , 16 :2479 , 2015 |
|
Abstract :
Iterative typeI polyketide synthases (PKSs) from fungi are multifunctional enzymes that use their active sites repeatedly in a highly ordered sequence to assemble complex natural products. A phytotoxic macrolide with anticancer properties, 10,11-dehydrocurvularin (DHC), is produced by cooperation of a highly reducing (HR) iterative PKS and a non-reducing (NR) iterative PKS. We have identified the DHC gene cluster in Alternaria cinerariae, heterologously expressed the active HR PKS (Dhc3) and NR PKS (Dhc5) in yeast, and compared them to corresponding proteins that make DHC in Aspergillus terreus. Phylogenetic analysis and homology modeling of these enzymes identified variable surfaces and conserved motifs that are implicated in product formation. |
| PubMedSearch : Cochrane_2015_Chembiochem_16_2479 |
| PubMedID: 26493380 |
| Gene_locus related to this paper: aspte-curs2 , altci-dhc5 |
| Gene_locus | aspte-curs2 altci-dhc5 |
Cochrane RV, Gao Z, Lambkin GR, Xu W, Winter JM, Marcus SL, Tang Y, Vederas JC (2015)
Comparison of 10,11-Dehydrocurvularin Polyketide Synthases from Alternaria cinerariae and Aspergillus terreus Highlights Key Structural Motifs
Chembiochem
16 :2479
Cochrane RV, Gao Z, Lambkin GR, Xu W, Winter JM, Marcus SL, Tang Y, Vederas JC (2015)
Chembiochem
16 :2479