| Title : A genome annotation-driven approach to cloning the human ORFeome - Collins_2004_Genome.Biol_5_R84 |
| Author(s) : Collins JE , Wright CL , Edwards CA , Davis MP , Grinham JA , Cole CG , Goward ME , Aguado B , Mallya M , Mokrab Y , Huckle EJ , Beare DM , Dunham I |
| Ref : Genome Biol , 5 :R84 , 2004 |
|
Abstract :
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined. |
| PubMedSearch : Collins_2004_Genome.Biol_5_R84 |
| PubMedID: 15461802 |
| Gene_locus related to this paper: human-SERHL2 |
| Gene_locus | human-SERHL2 |
Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I (2004)
A genome annotation-driven approach to cloning the human ORFeome
Genome Biol
5 :R84
Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I (2004)
Genome Biol
5 :R84