Comakli_2023_Chem.Biodivers__e202201231

In the study, water, ethanol, methanol, dichloromethane, and acetone extracts of Asparagus officinalis L. were obtained by maceration. DPPH , ABTS (+) , FRAP, and CUPRAC methods determined the antioxidant capacities of all extracts. Moreover, the invitro effects of extracts on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase (CA)-I, CA-II and alpha-Glycosidase were investigated. At a 10microg/ml concentration, the extract with the highest Fe(3+) reduction capacity was ethanol (AE), and the extract with the highest Cu(2+) reduction capacity was acetone (AA). AE for AChE (IC(50) =21.19microg/ml) and alpha-Glycosidase (IC(50) : 70.00microg/ml), methanol (AM) for BChE (IC(50) =17.33microg/ml), CA-I and II (IC(50) =79.65 and 36.09microg/ml, respectively) showed the most potent inhibition effect. The content analysis of acetone extract was performed with LC/MS-MS, the first three phytochemicals found most were p-Coumaric acid, rutin, and 4-hydroxybenzoic acid (284.29+/-3.97, 135.39+/-8.19, and 102.06+/-5.51microg analyte/g extract, respectively).