| Title : Response surface analysis for the production of an enantioselective lipase from Aspergillus niger by solid-state fermentation - Contesini_2009_J.Microbiol_47_563 |
| Author(s) : Contesini FJ , da Silva VC , Maciel RF , de Lima RJ , Barros FF , de Oliveira Carvalho P |
| Ref : J Microbiol , 47 :563 , 2009 |
| Abstract : The lipase produced by the Aspergillus niger strain AC-54 has been widely studied due to its enantioselectivity for racemic mixtures. This study aimed to optimize the production of this enzyme using statistical methodology. Initially a Plackett-Burman (PB) design was used to evaluate the effects of the culture medium components and the culture conditions. Twelve factors were screened: water content, glucose, yeast extract, peptone, olive oil, temperature, NaH(2)P0(4), KH(2)P0(4), MgS0(4)-7H(2)0, CaCl(2), NaCI, and MnS0(4). The screening showed that the significant factors were water content, glucose, yeast extract, peptone, NaH(2)P0(4), and KH(2)P0(4), which were optimized using response surface methodology (RSM) and a mathematical model obtained to explain the behavioral process. The best lipase activity was attained using the following conditions: water content (20%), glucose (4.8%), yeast extract (4.0%), and NaH2P04 (4.0%). The predicted lipase activity was 33.03 U/ml and the experimental data confirmed the validity of the model. The enzymatic activity was expressed as micromoles of oleic acid released per minute of reaction (micromol/min). |
| ESTHER : Contesini_2009_J.Microbiol_47_563 |
| PubMedSearch : Contesini_2009_J.Microbiol_47_563 |
| PubMedID: 19851729 |
Contesini FJ, da Silva VC, Maciel RF, de Lima RJ, Barros FF, de Oliveira Carvalho P (2009)
Response surface analysis for the production of an enantioselective lipase from Aspergillus niger by solid-state fermentation
J Microbiol
47 :563
Contesini FJ, da Silva VC, Maciel RF, de Lima RJ, Barros FF, de Oliveira Carvalho P (2009)
J Microbiol
47 :563