Correa_1998_J.Neurosci.Methods_84_93

A restraining box for the head and body of the electric fish Gymnotus carapo was constructed and coupled to a micromanipulator, permitting us to prepare an atlas of the telencephalon with stereotaxic parameters. A photograph and a schematic drawing of an animal's head is presented, showing two skin electroreceptors that were used as external landmarks. A sagittal section of the telencephalic structure is also presented, whose vertical bars indicate the frontal planes that compose the atlas. The frontal planes of the atlas consist of serial sections spaced 600 or 500 microns apart in the rostrocaudal axis. Sections mapped with acetylcholinesterase are shown, intercalated with Nissl-stained sections. The acetylcholinesterase sections proved to be useful for the delimitation of certain nuclei and for the exact localization of small fissures and fiber tracts. A brief description of major cytoarchitectural subdivision and connections of the telencephalon is provided.