Correa_2013_J.Appl.Microbiol_115_114

Reference

Title : Carboxyl ester hydrolase from Penicillium expansum: cloning, characterization and overproduction by Penicillium griseoroseum - Correa_2013_J.Appl.Microbiol_115_114
Author(s) : Correa TL , Zubieta MP , Teixeira JA , de Queiroz MV , de Araujo EF
Ref : J Appl Microbiol , 115 :114 , 2013
Abstract :

AIMS: In this study, a gene that encodes a carboxylesterase (carb) in Penicillium expansum GF was cloned, sequenced and overexpressed by Penicillium griseoroseum PG63, and the enzyme was characterized. METHODS AND RESULTS: The recombinant strain, P. griseoroseum T55, obtained upon transformation using the plasmid pAN-52-1-carb, showed integration of the carb gene into at least two heterologous sites of the genome by Southern blotting. Furthermore, the recombinant strain T55 exhibited almost a fourfold increase in carboxylesterase activity compared with PG63 strain when both were cultured without inducers. Based on the secondary structure and multiple sequence alignments with carboxylesterases, cholinesterase and lipase, a three-dimensional model was obtained. The alpha/beta barrel topology, that is typical of esterases and lipases, was indicated for the CARB protein with Ser(213)-Glu(341)-His(456) as the putative catalytic triad. CARB preferentially hydrolysed acyl chains with eight carbon atoms, and its activity was optimal at a pH of 7.0 and a temperature of 25 degreesC. CARB exhibited stability in alkaline pH, high activity under mesophilic conditions and stability in organic solvents. CONCLUSION: The CARB protein is potentially useful in bioremediation, food and chemical/pharmaceutical industries. SIGNIFICANCE AND IMPACT OF THE STUDY: This study is the first to report the development of a recombinant strain superproducing a Penicillium sp. carboxylesterase.

PubMedSearch : Correa_2013_J.Appl.Microbiol_115_114
PubMedID: 23581645
Gene_locus related to this paper: neofi-a1d634

Related information

Gene_locus neofi-a1d634

Citations formats

Correa TL, Zubieta MP, Teixeira JA, de Queiroz MV, de Araujo EF (2013)
Carboxyl ester hydrolase from Penicillium expansum: cloning, characterization and overproduction by Penicillium griseoroseum
J Appl Microbiol 115 :114

Correa TL, Zubieta MP, Teixeira JA, de Queiroz MV, de Araujo EF (2013)
J Appl Microbiol 115 :114