| Title : A novel sn-1,3 specific lipase from Janibacter sp. as catalysts for the high-yield synthesis of long-medium-long type structured triacylglycerols - Cui_2021_Food.Chem_366_130523 |
| Author(s) : Cui R , Xu L , Lan D , Yang B , Wang Y |
| Ref : Food Chem , 366 :130523 , 2021 |
|
Abstract :
Our study offers a novel sn-1,3 specific lipase MAJ1 from marine member Janibacter sp. strain HTCC2649 for preparing long-medium-long (LML) type structured triacylglycerols (TAGs). Firstly, the resin ECR1030 was selected as a suitable support for the immobilization of lipase MAJ1. An efficient synthesis of LML-type structured TAGs by the immobilized lipase MAJ1-catalyzed interesterification of methyl palmitate and tricaprylin was studied in a solvent-free system. The reaction conditions, including substrate molar ratio, temperature and enzyme loading, were optimized. Under the optimum conditions (immobilized lipase MAJ1 of 45 U/g, substrate molar ratio of 4:1, temperature of 35 degreesC, reaction time of 24 h), the structured TAGs with double long chains (DLCST) were obtained in a yield of 44.3 mol%. Secondly, multi-dimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) was employed to quantify each TAG positional isomer in DLCST. The content of 1,3-dipalmitoyl-2-capryloyl-sn-glycerol in DLCST was 97.6% determined by the MDMS-SL technology. |
| PubMedSearch : Cui_2021_Food.Chem_366_130523 |
| PubMedID: 34303206 |
| Gene_locus related to this paper: 9mico-a3tmr7 |
| Gene_locus | 9mico-a3tmr7 |
Cui R, Xu L, Lan D, Yang B, Wang Y (2021)
A novel sn-1,3 specific lipase from Janibacter sp. as catalysts for the high-yield synthesis of long-medium-long type structured triacylglycerols
Food Chem
366 :130523
Cui R, Xu L, Lan D, Yang B, Wang Y (2021)
Food Chem
366 :130523