Curtis_2012_Life.Sci_91_1070

Reference

Title : Nicotinic acetylcholine receptor stimulation impairs epidermal permeability barrier function and recovery and modulates cornified envelope proteins - Curtis_2012_Life.Sci_91_1070
Author(s) : Curtis BJ , Plichta JK , Blatt H , Droho S , Griffin TM , Radek KA
Ref : Life Sciences , 91 :1070 , 2012
Abstract :

AIM: To characterize how nicotinic acetylcholine receptors (nAChRs) influence epidermal barrier function and recovery following prolonged stress or direct nAChR activation or antagonism. MAIN
METHODS: Mice were subjected to psychological stress or treated topically with nAChR agonist or antagonist for 3 days. We assessed barrier permeability and recovery by measuring transepidermal water loss (TEWL) before and after barrier disruption. In parallel, we analyzed the production and localization of several epidermal cornified envelope proteins in mouse skin and in human EpiDerm organotypic constructs stimulated with a nAChR agonist (nicotine) and/or a nAChR selective antagonist (alpha-bungarotoxin). KEY FINDINGS: We determined that psychological stress in mice impairs barrier permeability function and recovery, an effect that is reversed by application of the alpha7 selective nAChR antagonist, alpha-bungarotoxin (Bung). In the absence of stress, both topical nicotine or Bung treatment alone impaired barrier permeability. We further observed that stress, topical nicotine, or topical Bung treatment in mice influenced the abundance and/or localization of filaggrin, loricrin, and involucrin. Similar alterations in these three major cornified envelope proteins were observed in human EpiDerm cultures. SIGNIFICANCE: Perceived psychological stress and nicotine usage can both initiate or exacerbate several dermatoses by altering the cutaneous permeability barrier. Modulation of nAChRs by topical agonists or antagonists may be used to improve epidermal barrier function in skin diseases associated with defects in epidermal barrier permeability.

PubMedSearch : Curtis_2012_Life.Sci_91_1070
PubMedID: 22940618

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Citations formats

Curtis BJ, Plichta JK, Blatt H, Droho S, Griffin TM, Radek KA (2012)
Nicotinic acetylcholine receptor stimulation impairs epidermal permeability barrier function and recovery and modulates cornified envelope proteins
Life Sciences 91 :1070

Curtis BJ, Plichta JK, Blatt H, Droho S, Griffin TM, Radek KA (2012)
Life Sciences 91 :1070