| Title : Validation of a microscale extraction and high-throughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia - Cuthbertson_2012_Biomed.Chromatogr_26_1191 |
| Author(s) : Cuthbertson D , Piljac-Zegarac J , Lange BM |
| Ref : Biomedical Chromatography , 26 :1191 , 2012 |
|
Abstract :
Herein we report on an improved method for the microscale extraction of huperzine A HupA an acetylcholinesterase-inhibiting alkaloid from as little as 3 mg of tissue homogenate from the clubmoss Huperzia squarrosa G Forst Trevis with 99.95 recovery We also validated a novel UHPLC-QTOF-MS method for the high-throughput analysis of H squarrosa extracts in only 6 min which in combination with the very low limit of detection 20 pg on column and the wide linear range for quantification 20-10,000 pg on column allow for a highly efficient screening of extracts containing varying amounts of HupA Utilization of this methodology has the potential to conserve valuable plant resources Copyright c 2012 John Wiley Sons Ltd. |
| PubMedSearch : Cuthbertson_2012_Biomed.Chromatogr_26_1191 |
| PubMedID: 22275140 |
Cuthbertson D, Piljac-Zegarac J, Lange BM (2012)
Validation of a microscale extraction and high-throughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia
Biomedical Chromatography
26 :1191
Cuthbertson D, Piljac-Zegarac J, Lange BM (2012)
Biomedical Chromatography
26 :1191