Czumaj_2019_Anticancer.Res_39_3815

Reference

Title : In Vivo Effectiveness of Orlistat in the Suppression of Human Colorectal Cancer Cell Proliferation - Czumaj_2019_Anticancer.Res_39_3815
Author(s) : Czumaj A , Zabielska J , Pakiet A , Mika A , Rostkowska O , Makarewicz W , Kobiela J , Sledzinski T , Stelmanska E
Ref : Anticancer Research , 39 :3815 , 2019
Abstract :

BACKGROUND/AIM: Fatty acid synthase (FASN) provides palmitate for cell membrane formation in colorectal cancer (CRC) cells, however, palmitate is also available in the blood of CRC patients. The aim of this study was to examine whether orlistat, a FASN inhibitor, is able to attenuate CRC cell growth despite the availability of extracellular palmitate. MATERIALS AND METHODS: Palmitate concentrations were measured in serum from CRC patients and healthy controls. HT-29 CRC cells were treated with orlistat and palmitate. RESULTS: Treatment of CRC cells with orlistat caused a dose-dependent inhibition of cell proliferation. In turn, delivery of extracellular palmitate at doses lower than those found in the serum of CRC patients reversed inhibition by orlistat concentrations of up to 10 muM. CONCLUSION: Inhibition of CRC cell proliferation by orlistat is reversed by palmitate which is present at high levels in the serum. Therefore, orlistat may be effective in vivo only at high concentrations.

PubMedSearch : Czumaj_2019_Anticancer.Res_39_3815
PubMedID: 31262909

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Citations formats

Czumaj A, Zabielska J, Pakiet A, Mika A, Rostkowska O, Makarewicz W, Kobiela J, Sledzinski T, Stelmanska E (2019)
In Vivo Effectiveness of Orlistat in the Suppression of Human Colorectal Cancer Cell Proliferation
Anticancer Research 39 :3815

Czumaj A, Zabielska J, Pakiet A, Mika A, Rostkowska O, Makarewicz W, Kobiela J, Sledzinski T, Stelmanska E (2019)
Anticancer Research 39 :3815