| Title : Synaptophysin regulates clathrin-independent endocytosis of synaptic vesicles - Daly_2000_Proc.Natl.Acad.Sci.U.S.A_97_6120 |
| Author(s) : Daly C , Sugimori M , Moreira JE , Ziff EB , Llinas R |
| Ref : Proc Natl Acad Sci U S A , 97 :6120 , 2000 |
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Abstract :
The GTPase dynamin I is required for synaptic vesicle (SV) endocytosis. Our observation that dynamin binds to the SV protein synaptophysin in a Ca(2+)-dependent fashion suggested the possibility that a dynamin/synaptophysin complex functions in SV recycling. In this paper we show that disruption of the dynamin/synaptophysin interaction by peptide injection into the squid giant synapse preterminal results in a decrease in transmitter release during high-frequency stimulation, indicating an inhibition of SV recycling. Electron microscopy of these synapses reveals a depletion of SVs, demonstrating a block of vesicle retrieval after fusion. In addition, we observed an increase in clathrin-coated vesicles, indicating that the peptide does not block clathrin-dependent endocytosis. We conclude that the dynamin/synaptophysin complex functions in a clathrin-independent mechanism of SV endocytosis that is required for efficient synaptic transmission. |
| PubMedSearch : Daly_2000_Proc.Natl.Acad.Sci.U.S.A_97_6120 |
| PubMedID: 10823955 |
Daly C, Sugimori M, Moreira JE, Ziff EB, Llinas R (2000)
Synaptophysin regulates clathrin-independent endocytosis of synaptic vesicles
Proc Natl Acad Sci U S A
97 :6120
Daly C, Sugimori M, Moreira JE, Ziff EB, Llinas R (2000)
Proc Natl Acad Sci U S A
97 :6120