Dasgupta_2005_J.Colloid.Interface.Sci_289_566

Reference

Title : Surfactant tail length-dependent lipase activity profile in cationic water-in-oil microemulsions - Dasgupta_2005_J.Colloid.Interface.Sci_289_566
Author(s) : Dasgupta A , Das D , Mitra RN , Das PK
Ref : J Colloid Interface Sci , 289 :566 , 2005
Abstract :

The catalytic activity of Chromobacterium viscosum lipase (CV-lipase) was estimated across varying surfactant tail lengths (C-10-C-18) in water-in-oil (w/o) microemulsions of cationic surfactants containing four different hydroxyethyl-substituted head groups. An attempt to find a correlation, if any, between the activity of interfacially solubilized lipase and the varying surfactant tails was made for the first time in micellar enzymology. The second-order rate constant, k2, in lipase-catalyzed hydrolysis of p-nitrophenyl-n-hexanoate at pH 6.0 and 25 degrees C shows an improvement in enzyme activity (approximately 30-140%) across different head groups of amphiphiles with increasing tail lengths in varying solution compositions. Improvement of enzyme activity is prominent in ascending from C-10 to C-14/C-16, depending on the nature of polar head group. The hydrolytic activity of lipase in different surfactant (50 mM)/water/isooctane/n-hexanol with varying z= [alcohol]/[surfactant] (6.4 or 4.8) was amplified by 25-250% with increment in surfactant tail length in comparison with widely used cationic w/o microemulsions having solution compositions (z=16). As a notable outcome of this research, we found w/o microemulsions of 25 mM tetradecyltrimethylammonium bromide/water/isooctane/n-hexanol (z=8) producing the highest ever activity of lipase in any w/o microemulsions.

PubMedSearch : Dasgupta_2005_J.Colloid.Interface.Sci_289_566
PubMedID: 16112238

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Citations formats

Dasgupta A, Das D, Mitra RN, Das PK (2005)
Surfactant tail length-dependent lipase activity profile in cationic water-in-oil microemulsions
J Colloid Interface Sci 289 :566

Dasgupta A, Das D, Mitra RN, Das PK (2005)
J Colloid Interface Sci 289 :566