Title : Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation - Daumann_2014_J.Inorg.Biochem_131_1 |
Author(s) : Daumann LJ , Larrabee JA , Ollis D , Schenk G , Gahan LR |
Ref : J Inorg Biochem , 131 :1 , 2014 |
Abstract :
Annually thousands of people die or suffer from organophosphate (pesticide) poisoning. In order to remove these toxic compounds from the environment, the use of enzymes as bioremediators has been proposed. We report here a Ser127Ala mutant based on the enzyme glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes. The mutant, with improved metal binding abilities, has been immobilized using glutaraldehyde on PAMAM dendrimer-modified magnetite nanoparticles. The immobilized system was characterized using elemental analysis as well as infrared, transmission electron and X-ray photoelectron spectroscopies. The amount of GpdQ that was immobilized with the optimized procedure was 1.488nmol per g MNP. A kinetic assay has been designed to evaluate the activity of the system towards organophosphoester substrates. The specific activity towards BPNPP directly after immobilization was 3.55mumolmg(-1)min(-1), after one week 3.39mumolmg(-1)min(-1) and after 120days 3.36mumolmg(-1)min(-1), demonstrating that the immobilized enzyme was active for multiple cycles and could be stored on the nanoparticles for a prolonged period. |
PubMedSearch : Daumann_2014_J.Inorg.Biochem_131_1 |
PubMedID: 24239906 |
Daumann LJ, Larrabee JA, Ollis D, Schenk G, Gahan LR (2014)
Immobilization of the enzyme GpdQ on magnetite nanoparticles for organophosphate pesticide bioremediation
J Inorg Biochem
131 :1
Daumann LJ, Larrabee JA, Ollis D, Schenk G, Gahan LR (2014)
J Inorg Biochem
131 :1