De Schrijver_1997_Appl.Environ.Microbiol_63_1911

Reference

Title : Thiocarbamate herbicide-inducible nonheme haloperoxidase of Rhodococcus erythropolis NI86\/21 - De Schrijver_1997_Appl.Environ.Microbiol_63_1911
Author(s) : De Schrijver A , Nagy I , Schoofs G , Proost P , Vanderleyden J , van Pee KH , De Mot R
Ref : Applied Environmental Microbiology , 63 :1911 , 1997
Abstract :

During biodegradation of thiocarbamate herbicides by Rhodococcus erythropolis NI86/21, a protein with an M(r) of 30,000 is induced (I. Nagy, G. Schoofs, F. Compernolle, P. Proost, J. Vanderleyden, and R.De Mot, J. Bacteriol. 177:676-687, 1995). Based on N-terminal sequence data for the protein purified by two-dimensional electrophoresis, the corresponding structural gene, thcF, was cloned and sequenced. The deduced protein sequence of ThcF is homologous to those of nonheme haloperoxidases. A particularly high level of sequence identity (72.6%) was observed for the chloroperoxidase from Pseudomonas pyrrocinia. A polyclonal antibody against the latter enzyme cross-reacted with ThcF either produced by the original Rhodococcus cells or overexpressed heterologously in Escherichia coli. In both thiocarbamate-grown Rhodococcus cells and E. coli cells expressing thcF, the haloperoxidase activity of ThcF was demonstrated. The thiocarbamate-inducible R. erythropolis ThcF protein represents the first (nonheme) haloperoxidase to be identified in a nocardioform actinomycete.

PubMedSearch : De Schrijver_1997_Appl.Environ.Microbiol_63_1911
PubMedID: 9143122
Gene_locus related to this paper: rhoer-thcf

Related information

Gene_locus rhoer-thcf

Citations formats

De Schrijver A, Nagy I, Schoofs G, Proost P, Vanderleyden J, van Pee KH, De Mot R (1997)
Thiocarbamate herbicide-inducible nonheme haloperoxidase of Rhodococcus erythropolis NI86\/21
Applied Environmental Microbiology 63 :1911

De Schrijver A, Nagy I, Schoofs G, Proost P, Vanderleyden J, van Pee KH, De Mot R (1997)
Applied Environmental Microbiology 63 :1911