| Title : Production of a thermostable extracellular lipase by Kluyveromyces marxianus - Deive_2003_Biotechnol.Lett_25_1403 |
| Author(s) : Deive FJ , Costas M , Longo MA |
| Ref : Biotechnol Lett , 25 :1403 , 2003 |
| Abstract : Kluyveromyces marxianus was grown in submerged culture in a complex medium with several potential inducers of lipolytic activity (triacylglycerols, fatty acids). The highest extracellular lipolytic enzyme production (about 80 U ml(-1) in 3 d) was obtained when the medium was supplemented with 2 g urea l(-1) plus 5 g tributyrin l(-1). Addition of surfactants (1 g l(-1)) did not improve production. The lipase had a high thermal stability in aqueous solution (73% residual activity after 9 d at 50 degrees C, 16 min half-life time at 100 degrees C). It was also stable at acidic pH and showed good tolerance to organic solvents (70% residual activity after 2 d in n-hexane of cyclohexane). |
| ESTHER : Deive_2003_Biotechnol.Lett_25_1403 |
| PubMedSearch : Deive_2003_Biotechnol.Lett_25_1403 |
| PubMedID: 14514040 |
Deive FJ, Costas M, Longo MA (2003)
Production of a thermostable extracellular lipase by Kluyveromyces marxianus
Biotechnol Lett
25 :1403
Deive FJ, Costas M, Longo MA (2003)
Biotechnol Lett
25 :1403