| Title : Lipolytic Activity of a Carboxylesterase from Bumblebee (Bombus ignitus) Venom - Deng_2021_Toxins.(Basel)_13_ |
| Author(s) : Deng Y , Kim BY , Lee KY , Yoon HJ , Wan H , Li J , Lee KS , Jin BR |
| Ref : Toxins (Basel) , 13 : , 2021 |
| Abstract : Bee venom is a complex mixture composed of peptides, proteins with enzymatic properties, and low-molecular-weight compounds. Although the carboxylesterase in bee venom has been identified as an allergen, the enzyme's role as a venom component has not been previously elucidated. Here, we show the lipolytic activity of a bumblebee (Bombus ignitus) venom carboxylesterase (BivCaE). The presence of BivCaE in the venom secreted by B. ignitus worker bees was confirmed using an anti-BivCaE antibody raised against a recombinant BivCaE protein produced in baculovirus-infected insect cells. The enzymatic activity of the recombinant BivCaE protein was optimal at 40 degreesC and pH 8.5. Recombinant BivCaE protein degrades triglycerides and exhibits high lipolytic activity toward long-chain triglycerides, defining the role of BivCaE as a lipolytic agent. Bee venom phospholipase A(2) binds to mammalian cells and induces apoptosis, whereas BivCaE does not affect mammalian cells. Collectively, our data demonstrate that BivCaE functions as a lipolytic agent in bee venom, suggesting that BivCaE will be involved in distributing the venom via degradation of blood triglycerides. |
| ESTHER : Deng_2021_Toxins.(Basel)_13_ |
| PubMedSearch : Deng_2021_Toxins.(Basel)_13_ |
| PubMedID: 33810599 |
Deng Y, Kim BY, Lee KY, Yoon HJ, Wan H, Li J, Lee KS, Jin BR (2021)
Lipolytic Activity of a Carboxylesterase from Bumblebee (Bombus ignitus) Venom
Toxins (Basel)
13 :
Deng Y, Kim BY, Lee KY, Yoon HJ, Wan H, Li J, Lee KS, Jin BR (2021)
Toxins (Basel)
13 :