| Title : Quantification of intra- and extra-cellular thermophilic lipase\/esterase production by Thermus sp - Dominguez_2004_Biotechnol.Lett_26_705 |
| Author(s) : Dominguez A , Sanroman A , Fucinos P , Rua ML , Pastrana L , Longo MA |
| Ref : Biotechnol Lett , 26 :705 , 2004 |
|
Abstract :
Four Thermus strains produced lipolytic activity when grown in liquid medium for 30 h at 70 degrees C. The highest total lipase/esterase activity (57 U l(-1)) was in Thermus aquaticus YT-1, followed by Thermus thermophilus HB27 and HB8 (33 and 25 U l(-1), respectively), and finally by Thermus sp. (16 U l(-1)). Extra-cellular activity was detected in T. aquaticus YT-1 and T. thermophilus HB27 (33 and 17 U l(-1)). All enzymes were stable at 80 degrees C over 30 min, and their activity towards fatty acid esters increased as substrate chain-length diminished (i.e. hydrolysis rate was up to 6-fold higher on p-nitrophenyl caproate than on laurate). |
| PubMedSearch : Dominguez_2004_Biotechnol.Lett_26_705 |
| PubMedID: 15195968 |
Dominguez A, Sanroman A, Fucinos P, Rua ML, Pastrana L, Longo MA (2004)
Quantification of intra- and extra-cellular thermophilic lipase\/esterase production by Thermus sp
Biotechnol Lett
26 :705
Dominguez A, Sanroman A, Fucinos P, Rua ML, Pastrana L, Longo MA (2004)
Biotechnol Lett
26 :705