Dong_2014_World.J.Microbiol.Biotechnol_30_1011

Reference

Title : Indentification of huperzine A-producing endophytic fungi isolated from Huperzia serrata - Dong_2014_World.J.Microbiol.Biotechnol_30_1011
Author(s) : Dong LH , Fan SW , Ling QZ , Huang BB , Wei ZJ
Ref : World J Microbiol Biotechnol , 30 :1011 , 2014
Abstract :

This present study was designed to investigate the production of huperzine A (HupA), an acetylcholine inhibitor, which was produced by an endophytic fungi isolated from Huperzia serrata. Screening of 94 endophytic fungal isolates obtained from plant H. serrata was carried out for the production of HupA. Their morphological characteristics were studied and rDNA sequence analysis was carried out. The cultures were grown in liquid culture medium and the extracted metabolites were analyzed by thin layer chromatography and high performance liquid chromatograph for the presence of HupA. The DPPH scavenging ratio and inhibition ratio of acetylcholinesterase (AchE) of the same were determined. 3 out of 94 strains i.e. S29, L44 and S94 showed significant AchE-inhibitory activity and antioxidant activity. Strain L44 which exhibited maximum yield of HupA (37.63 mug/g on dry weight basis) was identified as Trichoderma species by ITS sequence analysis. In conclusion, endophytic fungi from H. serrata can be used as a new resource of HupA.

PubMedSearch : Dong_2014_World.J.Microbiol.Biotechnol_30_1011
PubMedID: 24129696

Related information

Citations formats

Dong LH, Fan SW, Ling QZ, Huang BB, Wei ZJ (2014)
Indentification of huperzine A-producing endophytic fungi isolated from Huperzia serrata
World J Microbiol Biotechnol 30 :1011

Dong LH, Fan SW, Ling QZ, Huang BB, Wei ZJ (2014)
World J Microbiol Biotechnol 30 :1011