Doods_1986_Naunyn.Schmiedebergs.Arch.Pharmacol_333_182

Reference

Title : Discrimination by N-ethylmaleimide between the chronotropic and inotropic response to muscarinic receptor stimulation in rat atrium - Doods_1986_Naunyn.Schmiedebergs.Arch.Pharmacol_333_182
Author(s) : Doods HN , Davidesko D , Mathy MJ , Batink HD , de Jonge A , van Zwieten PA
Ref : Naunyn Schmiedebergs Arch Pharmacol , 333 :182 , 1986
Abstract :

N-ethylmaleimide (NEM) rapidly blocked the negative chronotropic effect of carbachol on rat right atrium. In contrast, NEM did not reduce the negative inotropic response to muscarinic (M) receptor stimulation. Carbachol inhibited the specific binding of [3H]-N-methylscopolamine [( 3H]-NMS) to membranes of rat atria as reflected by a shallow inhibition curve. Both guanosine triphosphate (GTP) and NEM shifted the [3H]-NMS inhibition curves of carbachol to the right. Pretreatment of the atrial membranes with NEM abolished the GTP-induced rightward shift. However, when instead of the membranes the intact atria were pre-incubated with NEM, no interaction between NEM and GTP in the membranal preparation was observed. The results indicate that NEM sharply discriminated between the inotropic and chronotropic effects to M-receptor stimulation in rat atria. The inhibitory effect of NEM on the M-receptor-mediated negative chronotropic effect in rat atrium cannot be explained by an interaction of the sulfhydryl reagent with GTP-binding proteins, like Ni or No.

PubMedSearch : Doods_1986_Naunyn.Schmiedebergs.Arch.Pharmacol_333_182
PubMedID: 3748199

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Citations formats

Doods HN, Davidesko D, Mathy MJ, Batink HD, de Jonge A, van Zwieten PA (1986)
Discrimination by N-ethylmaleimide between the chronotropic and inotropic response to muscarinic receptor stimulation in rat atrium
Naunyn Schmiedebergs Arch Pharmacol 333 :182

Doods HN, Davidesko D, Mathy MJ, Batink HD, de Jonge A, van Zwieten PA (1986)
Naunyn Schmiedebergs Arch Pharmacol 333 :182