| Title : Acetylcholine biosensor involving entrapment of acetylcholinesterase and poly(ethylene glycol)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane - Doretti_2000_Enzyme.Microb.Technol_27_279 |
| Author(s) : Doretti L , Ferrara D , Lora S , Schiavon F , Veronese FM |
| Ref : Enzyme Microb Technol , 27 :279 , 2000 |
|
Abstract :
A bienzymatic sensor for the determination of acetylcholine was prepared by physical coimmobilization of acetylcholinesterase and poly(ethylene glycol)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane obtained by a cyclic freezing-thawing process. The enzyme-modified polymer was applied on a platinum electrode to form an amperometric sensor, based on the electrochemical detection of enzymatically developed hydrogen peroxide. The analytical characteristics of this sensor, including calibration curves for choline and acetylcholine, pH, and temperature effects, and stability are described. |
| PubMedSearch : Doretti_2000_Enzyme.Microb.Technol_27_279 |
| PubMedID: 10899554 |
Doretti L, Ferrara D, Lora S, Schiavon F, Veronese FM (2000)
Acetylcholine biosensor involving entrapment of acetylcholinesterase and poly(ethylene glycol)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane
Enzyme Microb Technol
27 :279
Doretti L, Ferrara D, Lora S, Schiavon F, Veronese FM (2000)
Enzyme Microb Technol
27 :279